We are pleased to announce that, after acquiring the sequencer early this summer, last week our wet lab successfully prepared and sequenced the lambda control genome on the PromethION P2 Solo, making long read sequencing with Oxoford Nanopore Technologies a reality at Wales Gene Park.

Our flow cell check showed the presence of 6848 pores and started running with a 93.3% pore occupancy. Here are some of the metrics of our run after 65 hours and one nuclease flush:

• 5.1 M reads
• N50 32.5 Kb
• 99.99 % basecalled reads
• Total data produced 985.8 Gb

All the PromethION specs can be found here.

At Wales Gene Park, we are looking forward to provide Nanopore sequencing services for cancer research in Wales in the future.

Please, get in touch at WalesGenePark@cardiff.ac.uk to know more about the PromethION P2 Solo and its applications to cancer research.