NGS Gwasanaeth

Ansicr am y ffordd orau o fynd ymlaen? Cysylltwch â’n staff labordy i ddod o hyd i lif gwaith a deilwrir i’ch nodau ymchwil WalesGenePark@cardiff.ac.uk

Paratoi llyfrgell NGS

Gall ein staff roi cymorth parhaus ar bob cam o’r biblinell genomeg, gan gynnwys echdynnu DNA, paratoadau llyfrgell DNA/RNA, llyfrgell ansawdd data (QC), dilyniannu Illumina a data dilyniannodi DNA y genhedlaeth nesaf (NGS). Dyma rai o’r llifoedd gwaith y mae ein staff yn eu gweithredu fel mater o drefn wrth baratoi llyfrgell NGS:

  • HaloPlex HS Target Enrichment System (Agilent Technologies)
  • Illumina DNA PCR-Free Library Prep, Tagmentation (Illumina)
  • Illumina DNA Prep Guide (Illumina)
  • TruSeq Exome Library Prep (Illumina)
  • TruSeq Methyl Capture EPIC Library Prep (Illumina)
  • NEBNext Ultra II DNA Library Prep kit for Illumina (BioLabs)
  • Nextera DNA Sample Preparation (Illumina)
  • Nextera Rapid Capture Enrichment (Illumina)
  • MicrobExpress Kit (Life Technologies)
  • TruSeq Stranded Total RNA (Illumina)
  • NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (BioLabs)
  • NEBNext Globin & rRNA Depletion Kit (BioLabs)
  • NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (BioLabs)
  • TruSeq Stranded mRNA Sample Preparation (Illumina)
  • NEBNext Multiplex Small RNA Library Prep Set for Illumina (BioLabs)
  • TruSeq Small RNA Library Prep (Illumina)
  • TruSeq ChIP Sample Preparation (Illumina)

Lab rhag-PCR Parc Geneteg Cymru yn adeilad Syr Geraint Evans, Heath.

Lab ar ol-PCR Parc Geneteg Cymru yn adeilad Syr Geraint Evans, Heath.

Llwyfannau dilyniant Illumina

    • MiSeq
    • NextSeq 550
    • NovaSeq 6000

Illumina NovaSeq 6000, NextSeq 550 a MiSeq ar safle ysbity Prifysgol Caerdydd.

Gwasanaethau eraill ar gael ar Parc Geneteg Cymru:

  • Covaris – Ultrasonicator (DNA/RNA darnio mecanyddol).
  • Meintioli DNA/RNA yn fanwl gywir ar gyfer dilniannodi DNA y genhedlaeth nesaf (NGS).
  • Nodweddu llyfregell, DNA/RNA HS a phecyn safonol (TapeStation, Agilent).